HOME > mice > B6N lox-Booster-PKAchu mice
Strain Detail: B6N lox-Booster-PKAchu mice
| Strain information |
| Resource No. |
nbio371 |
| Strain name |
B6N lox-Booster-PKAchu |
| Strain Nomenclature |
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| Common name/Synonyms |
lox-Booster-PKAchu |
| Strain types |
mutant |
| Background strain |
C57BL/6N |
| Institution |
Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University |
| Developer |
Michiyuki Matsuda |
| Depositor |
Michiyuki Matsuda |
| Conditions of distribution |
It's necessary to obtain depositor's consent on use of biological resources. contact us |
| Animal Health Report |
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| Strain description |
Transgenic mice expressing a probe with lox-mCherry-NLS added upstream of a cytoplasmically-localized FRET biosensor (Booster-PKA) that monitors the kinase activity of PKA. The mice with mCherry-NLS recombination induced by crossing with EIIa-Cre (+/+) mice systemically express Booster-PKA. Background strain heterozygous for the Tyrc gene is a hybrid between wild-type B6N and B6N Albino mice. |
| Reference(s) |
- Watabe T, Terai K, Sumiyama K, Matsuda M. (2020) Booster, a Red-Shifted Genetically Encoded Förster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools. ACS Sens. 5:719-730. [PMID: 32101394]
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| Resource information |
| Cryopreserved sperm |
In-house |
Genotype |
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| Medium |
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| From other institutions |
Genotype |
heterozygous |
| Medium |
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| Cryopreserved embryo |
In-house |
Mating System |
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| Genotype |
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| Medium |
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| From other institutions |
Mating System |
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| Genotype |
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| Method |
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| Strain status / Availability |
Cryopreserved sperm |
Within 1 month |
| Cryopreserved embryo |
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| Live animals |
Approx. 2 months |
| Gene information |
| Mutation types |
transgenic |
| Gene symbol |
mCherry, mKate2, mKOκ |
| Gene name |
red, red, and orange fluorescent proteins. |
| Genotyping information |
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