HOME > mice > FVB.lox-Eisuke-NES mice
Strain Detail: FVB.lox-Eisuke-NES mice
| Strain information | |
|---|---|
| Resource No. | nbio274 |
| Strain name | FVB.lox-Eisuke-NES |
| Strain Nomenclature | FVB.Tg(lox-EKAREV)pT2A-lox-tdKeima-3903NES |
| Common name/Synonyms | |
| Strain types | mutant |
| Background strain | FVB/N |
| Institution | Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University |
| Developer | Michiyuki Matsuda |
| Depositor | Michiyuki Matsuda |
| Conditions of distribution | It's necessary to obtain depositor's consent on use of biological resources.Contact us |
| Animal Health Report | |
| Strain description | FVB.lox-Eisuke-NES mice are transgenic mice expressing cytoplasm-localized förster (or fluorescence) resonance energy transfer (FRET) biosensor monitoring ERK kinase activity with lox-tdKeima. |
| Reference(s) |
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| Resource information | |||
|---|---|---|---|
| Cryopreserved sperm | In-house | Genotype | Hemizygous |
| Medium | FERTIUP | ||
| From other institutions | Genotype | ||
| Medium | |||
| Cryopreserved embryo | In-house | Mating System | IVF |
| Genotype | mix of hemizygous and wild-type | ||
| Medium | EFS40 | ||
| From other institutions | Mating System | ||
| Genotype | |||
| Method | |||
| Strain status / Availability | Cryopreserved sperm | Within 1 month | |
| Cryopreserved embryo | Within 1 month | ||
| Live animals | Approx. 2 months | ||
| Gene information | |
|---|---|
| Mutation types | transgenic |
| Gene symbol | CFP, YFP, Keima |
| Gene name | Cyan Fluorescent Protein, Yellow Fluorescent Protein, Keima |
| Genotyping information | The presence of transgenes can be confirmed by red fluorescence (~620nm, Keima's color) from body surface under blue light (~450nm). Note that it may be difficult to detect red fluorescence in new born pups just after the delivery. It is easier to detect |