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Strain Detail: Tmppe-KO mice
| Strain information |
| Resource No. |
nbio416 |
| Strain name |
Tmppe-KO |
| Strain Nomenclature |
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| Common name/Synonyms |
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| Strain types |
mutant |
| Background strain |
C57BL/6N |
| Institution |
Laboratory of Animal Models for Human Diseases, National Institutes of Biomedical Innovation, Health and Nutrition |
| Developer |
Osamu Suzuki |
| Depositor |
Osamu Suzuki |
| Conditions of distribution |
Collaboration with the depositor only. It's necessary to obtain depositor's consent on use of biological resources. contact us |
| Animal Health Report |
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| Strain description |
 Our laboratory has used knockout (KO) mice (BKO) of the Glb1 gene as a mouse model for GM1 gangliosidosis. The intron of the Glb1 gene contains the Tmppe gene; however, little is known about the properties of Tmppe. Therefore, Tmppe KO mice were generated by removing the entire coding sequence of the Tmppe gene by gene editing using the CRISPR/Cas9 system. The KO mice lost their TMPPE protein expression (see Western blot figure) without any apparent abnormality. This strain is useful for the study of TMPPE protein function. |
| Reference(s) |
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| Resource information |
| Cryopreserved sperm |
In-house |
Genotype |
Heterozygous |
| Medium |
Fertiup |
| From other institutions |
Genotype |
|
| Medium |
|
| Cryopreserved embryo |
In-house |
Mating System |
Heterozyous x B6NCr Slc |
| Genotype |
Heterozygous or wild |
| Medium |
EFS40 |
| From other institutions |
Mating System |
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| Genotype |
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| Method |
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| Strain status / Availability |
Cryopreserved sperm |
Within 1 month |
| Cryopreserved embryo |
Within 1 month |
| Live animals |
Approx. 2 months |
| Gene information |
| Mutation types |
knockout |
| Gene symbol |
Tmppe |
| Gene name |
transmembrane protein with metallophosphoesterase domain |
| Genotyping information |
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