HOME > mice > B6N lox-synPCB-Blvra−/− mice

Strain Detail: B6N lox-synPCB-Blvra−/− mice

Strain information
Resource No. nbio452
Strain name B6N lox-synPCB-Blvra−/−
Strain Nomenclature
Common name/Synonyms Blvra−/− SynPCB, B6 Albino-KOBACHI
Strain types mutant
Background strain B6 Albino
Institution Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University
Developer Michiyuki Matsuda
Depositor Michiyuki Matsuda
Conditions of distribution It's necessary to obtain depositor's consent on use of biological resources. contact us
Animal Health Report
Strain description

This strain expresses the ficocyanin bilin (PCB) synthase (SynPCB) gene in a Cre-dependent manner. It also lacks biliverdin reductase (Blvra, see B6N Albino-Blvra-/-), an enzyme that breaks down PCBs, and therefore accumulates high levels of PCBs.
Reference(s)
  1. Uda Y, Miura H, Goto Y, Yamamoto K, Mii Y, Kondo Y, Takada S, and Aoki K. (2020) Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics. ACS Chem Biol 15(11):2896-2906. [PMID:33164485]
Resource information
Cryopreserved sperm In-house Genotype lox-synPCB: hemizygous , Blvra: homozygous
Medium Fertiup
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System ♂:lox-synPCB: hemizygous / Blvra : homozygous x ♀: fluorescence (-) / Blvra: homozygous
Genotype lox-synPCB: hemizygous or wild, Blvra: homozygous
Medium EFS40
From other institutions Mating System
Genotype
Method
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types conditional KO
Gene symbol
Gene name
Genotyping information

Genotypes are determined by fluorescence on the body surface. The lox-synPCB mice express mCherry fluorescence throughout the body. Under green light (around 560 nm), mice with mCherry fluorescence (around 610 nm) are Blvra-/- SynPCB mice, and mice without mCherry fluorescence are Blvra-/- mice. If the mice have Cre enzymes, the red fluorescent protein is replaced by the blue fluorescent protein.

container -->