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Strain Detail: C57BL6/J (MHC-MCM-V2K) mice

Strain information
Resource No. nbio312
Strain name C57BL6/J (MHC-MCM-V2K)
Strain Nomenclature
Common name/Synonyms MHC-MCM-V2K
Strain types mutant
Background strain C57BL/6J
Institution National Cerebral and Cardiovascular Center
Developer Yuko Iwata
Depositor Yuko Iwata
Conditions of distribution Only for collaboration with the depositor: contact us
Animal Health Report
Strain description

Transient receptor potential cation channel (TRP), subfamily V, member 2 (TRPV2) is a Ca2+-permeable TRP channel. Its pathophysiological roles are still unknown. The C57BL/6J(MHC-MCM-V2K) strain is a hybrid of B6.FVB(129)-A1cfTg(Myh6-cre/Esr1*)1Jmk/J (a.k.a., αMHC-MerCreMer) and V2K mice. The myosin heavy chain promoter drives heart-specific expression of tamoxifen-dependent Cre recombinases in the C57BL/6J(MHC-MCM-V2K) mice. Tamoxifen treatment to this strain activates these heart-specific Cre recombinases, which silence TRPV2 expression in cardiac muscles by removing floxed alleles (exons from 3 to 5) of the Trpv2 gene. The strain is useful for pathophysiological analyses of TRPV2 in cardiac muscles.
Reference(s)
Resource information
Cryopreserved sperm In-house Genotype MHC-MCM（＋/W) V2(fl/fl)　
Medium FERTIUP
From other institutions Genotype
Medium
Cryopreserved embryo In-house Mating System IVF
Genotype heterozygous and wild-type for MHC-MCM, heterozygous for V2
Medium EFS40
From other institutions Mating System
Genotype
Method
Strain status / Availability Cryopreserved sperm Within 1 month
Cryopreserved embryo Within 1 month
Live animals Approx. 2 months
Gene information
Mutation types transgenic + conditional KO
Gene symbol MerCreMer, Trpv2
Gene name tamoxifen-inducible Cre recombinase, transient receptor potential cation channel, subfamily V, member 2
Genotyping information